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. 2006 Sep 20;34(18):5060–5068. doi: 10.1093/nar/gkl659

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Three leucine-rich sequences mediate cytoplasmic localization of MoKA. (A) At the top is a schematic representation of MoKA protein structure showing the relative locations of the three putative leucine-rich NESs identified by sequence homology. Below is the amino acid alignment of the four NES-like motifs. (B) Schematic representation of the MoKA-Flag mutants without NES-like sequences (ΔNESs). (C) Illustrative confocal images showing indirect immunofluorescence of COS7 cells transiently expressing MoKA-Flag wild-type (WT) and MoKAΔNESs-Flag fusion proteins. The bar graphs on the right summarize the individual N/C ratios of full-length MoKA-Flag (WT) and of various MoKAΔNESs-Flag fusion proteins.