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NESs2–4 counteract nuclear localization by the NLS of GAL4. Illustrative confocal images of indirect immunofluoresece of COS7 cells transiently expressing (1–147)GAL4 alone or fused to various NES peptides of MoKA. The bar graphs at the bottom summarize the individual N/C ratios of GAL4 and MoKA-NESs-GAL4 fusion products.
