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. 2006 Sep 20;34(18):5060–5068. doi: 10.1093/nar/gkl659

Figure 5.

Figure 5

CRM1-dependent and independent pathways mediate cytoplasmic accumulation of MoKA. (A) On the left are illustrative confocal image showing COS7 cells transiently expressing wild-type NES2 or the L194A/L196A mutant (NES2-WT and NES2mut) fused to GFP. The bar graphs on the right summarize the individual N/C ratios of NES2-WT, NES2mut and GFP. (B) Illustrative images of COS7 cells transiently expressing the wild-type (203/318)MoKA peptide (NES3 WT) and NES3b-mut, NES3c-mut, NES3b,c-mut1, NES3b,c-mut2 mutants fused to GFP. The bar graphs at the bottom right summarize the individual N/C ratios of GFP, and of wild-type and mutants NES3 fusion products.