Figure 6.

Retrotransposition frequency (RF) in mutants of the putative internal loop region of the UnaL2 3′ tail. The retrotransposition assay was performed as in Figure 5B and 5C. RFs were calculated as described in Materials and Methods. RFs and relative RF values (percentages) for mutants compared with the wild type 3′ tail of UnaL2 are shown. Two different experiments were performed for each construct and average values are shown. Images show each 100 mm plate with G-418-resistant colonies selected from ∼1.5–2.5 × 10⁶ hygromycin-resistant cells. UnaL2 tail (WT), ZfL2-2 having the wild type 3′ tail of UnaL2. No tail, ZfL2-2 having no conserved 3′ tail. C8A, C8U, C8A, C8del, C8CC and U28A: ZfL2-2 constructs having a mutation in the 3′ tail of UnaL2.