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. 2006 Sep 20;34(18):5093–5100. doi: 10.1093/nar/gkl670

Figure 2.

Figure 2

Cleavage/religation equilibrium of the CL25/CP25 fully duplex DNA substrate. Gel electrophoresis of the products coming from the incubation of the wild-type topoisomerase I with the [γ-32P] end-labelled duplex DNA, shown at the top of the figure in the absence (lane 1) and presence (lanes 2–6) of increasing amount of CPT. The arrow at the DNA sequence indicates the CL1 site preferred by the wild-type protein. Lanes 7 and 8–12 show the same experiment with the Glu418Lys mutant. The asterisk indicates the band corresponding to the CL1 site.