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. 2006 Sep 20;34(18):5093–5100. doi: 10.1093/nar/gkl670

Figure 5.

Figure 5

Religation experiment with the R11 oligonucleotide for the wild-type and Glu418Lys topoisomerase I. (A) Time course of the religation experiment between the R11 substrate and the wild-type or Glu418Lys covalent complexes, in the absence or presence of 100 µM CPT. The R11 oligonucleotide is selectively religated to the CL1 site but not to the CL2 site. (B) Percentage of the remaining covalent complex 1 plotted at different time for the wild-type and Glu418Lys (black and red lines, respectively), in absence (full line) and presence (dashed line) of CPT. Note the difference in scale between this figure and Figure 4. Three different religation experiments were averaged for each data point, and the error bars indicate the SD of the individual values from the mean.