Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cleavage/religation equilibrium of a 900 bp fully duplex DNA substrate. Gel electrophoresis of the products coming from the incubation of a 900 bp 32P-end-labelled DNA duplex with the wild-type topoisomerase I (lanes 1–5) or Glu418Lys mutant (lanes 6–10), as a function of time, in the absence (lanes 5 and 10) or in the presence of 1 µM CPT (lanes 1–4 and 6–9). Control, no enzyme added (C). Arrow indicates the preferred cleavage site.
