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. 2006 Oct 5;34(19):5603–5612. doi: 10.1093/nar/gkl619

Figure 2.

Figure 2

Detection of p53 mRNA abnormalities in SK-N-AS (AS) in comparison with SH-SY5Y (SH) cells. (A) Amplification of p53 cDNA using primers from exons 8 to 10 as indicated below each arrow; for precise position see Table 1 and GenBank K03199: F2 (forward primer in exon 8); R3 (reverse primer at the junction of exon 8/9); R4 (the first moiety of exon 9); R5 (exon 9, 30 nt downstream R4); R6 (beginning of exon 10). Note that no amplification was observed in SK-N-AS from exon 10 (last lane), in contrast to SH-SY5Y. (B) RT–PCR from SK-N-AS compared to SH-SY5Y cells. Specific primers (Table 1) were used to amplify the DBD, the p53β isoform (β) and the C-terminal domain (C-ter).