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. 2006 Oct 5;34(19):5603–5612. doi: 10.1093/nar/gkl619

Figure 4.

Figure 4

p53 transactivation ability using yeast-based assay (FASAY). (A) Schematic representation of the analysis of p53 mutants using the yeast homologous recombination expression vector pRDI-22 carrying the 5′ and 3′ ends of the p53 open reading frame and the split forms, pFW35 and pFW34 (lacking p53 fragment from amino acids 66 to 210 for split 5′ and 211–348 for split 3′, respectively) transfected into YPH500 Ade2 yeast strain. This strain repairs double-strand breaks in transfected plasmids by homologous recombination as ‘gap repair’ (see text). (B) Photographs of yeast colonies showing 100% wild-type p53 where all colonies are white (a), or special mutated p53 by duplication of exons 7–9 found in IGR-N-91 cells (b), where white and red colonies were mixed (see also Table 2), and mutated p53 such as those in SK-N-BE(2), where all colonies are red (c).