Skip to main content
. 2006 Oct 5;34(19):5603–5612. doi: 10.1093/nar/gkl619

Figure 6.

Figure 6

Western blot showing induction of p21/WAF1 protein by plasmid-recombinant expression vector of p53 variant transfected into p53-negative LAN-1 cells. LAN-1 cells were seeded onto 6-well plates. At a density of ∼60%, confluence cells were transfected with recombinant vector using Lipofectamine 2000 reagent according to the supplier's instructions (Invitrogen). To ascertain the transfection efficiency, cells were transfected in parallel experiments with pEGFP-C1 vector (Promega). The empty vector was used as a control. As shown in Figure 1, note that the p53 protein from the IGR-N-91 cells analyzed by SDS–PAGE migrated more slowly than the wild-type p53 due to duplication of exons 7-8-9 as described previously (13).