Figure 1.

Gene expression during growth of S.coelicolor strain M145. Spores were inoculated into R5 liquid medium and grown at 30°C for 3 days. The culture was monitored throughout the experiment for growth and production of the two pigmented antibiotics Act and Red (5; upper). Mycelium was harvested at the three time points indicated [early growth phase (T1), transition phase (T2) and stationary phase (T3)] to generate RNA samples for expression analysis by qrt-PCR (lower) and for use in subsequent DNase I-sensitivity experiments.