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Inter RNAP–RNAP separation distributions along the DNA contour length. RNAP centre separation: (A) in the absence of all NTPs (n = 45), i.e. OPCs; (B) in the presence of ATP, GTP and UTP only (n = 131), i.e. SECs; (C) in the presence of ATP, GTP and CTP, followed by CTP after 20 min (n = 44); (D) formed by addition of all 4 NTPs together (n = 45) from the OPC state.
