Table 3.
Comparison of steady-state levels of specific mRNAs in a PAP I-induced strain [SK9124, 90-fold increase in the total intracellular poly(A) level] compared to the wild-type control (MG1693) as determined by DNA macroarray and northern blot analysis
| Transcripta | Fold change in steady-state transcript levels | |
|---|---|---|
| Array data | Northern datab | |
| lpp (1) | +1.3 | +1.1 ± 0.1 |
| rpsO (1) | −1.1 | 1 ± 0.1 |
| ompA (1) | −1.4 | +1.1 ± 0.1 |
| trxA (1) | +1.4 | +1.1 ± 0.2 |
| rnc (1) | +1.4 | +1.2 ± 0.2 |
| rnb (1) | +1.2 | +1.1 ± 0.1 |
| pnp (2) | +2.4 | +2.5 ± 0.5c |
| rne (2) | +5.1 | +10 ± 2c |
| cspA (2) | +2.5 | +2.5 ± 0.5 |
| crcA (2) | +2.1 | +1.8 |
| cspE (3) | −2.7 | −2.4 ± 0.5 |
| yfiA (3) | −3.0 | −2.9 ± 0.5 |
| gltA (3) | −7.0 | −4.0 ± 1 |
| deoC (3) | −6.0 | −2.4 ± 0.2 |
aNumbers in parenthesis refer to categories defined in text.
bAll Northern analysis data represent the average of two to three independent determinations with the exception of crcA which was done only once.
cData derived from Ref. (23).