Figure 4.

Five sequential domains I, II, III (active domain), IV, and V of β-galactosidase (PDB ID: 1DP0), with starting residue positions given (42). For each of the oligos in the two test sets, either a ‘+’ indicating agreement between prediction and observed inhibition, or a ‘−’ for disagreement is placed under the protein residue position corresponding to the target site on the lacZ mRNA. Here, prediction and observed inhibition are considered to be in agreement if either the oligo has the observed activity ≥50% and its ΔG_disruption ≥ −7 kcal/mol, or else the oligo has the observed activity < 50% and its ΔG_disruption < −20 kcal/mol. For the 20 oligos in the two test sets, agreement is observed for 12 of the 14 oligos (86%) targeted to domains I, II and III. In contrast, agreement is only observed for two of the six oligos (33%) targeted to domains IV and V.