Table 1.
Oligonucleotides used in VITA protocol
| Oligo | Sequence | Description |
|---|---|---|
| bOC | b-AAAAAAAAAATACTGACGCTCTCGCACCCATCTCT | Position 383–407 on HIV genome, linear amplification of junctions |
| PVE | TCTCGCACCCATCTCTCTCC | Position 379–398 on HIV genome, amplification of junctions fragments |
| PVI | TGGTTTCCCTTTCGCTTTCA | Position 255–274 on HIV genome, amplification of junctions fragments |
| OLS | TCGGAGAAGAGGATACGGATTGTAGGCAGGGGGACGCCTCAAGAATAAGGGCT-a | |
| OLL | CCCTTATTCTTGAGGCGTCCCCCTGCCTACAATCCGTATCCTCTTCTCCGACATG | |
| PLE | CCTTATTCTTGAGGCGTCCC | |
| PLI | GGCGTCCCCCTGCCTACAAT | |
| bPLN | b-CGACGAGACACTGCCCCTGA | |
| bPLI | b-GTCCCCCTGCCTACAATCCG | |
| LNS | AACATGTTTGAATCTGTTTCAGGGGCAGTGTCTCGTCGGGA-a | |
| LNL | GAGTCCCGACGAGACACTGCCCCTGAAACAGATTCAAACATGTTNN |
b = biotin, a = amine.