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. 2006 Oct 5;34(19):5613–5622. doi: 10.1093/nar/gkl717

Figure 2.

Figure 2

(A) Positions of the ELVd (+) hammerhead covered by two antisense oligodeoxyribonucleotides (in color). Other details as in the legend to Figure 1. (B) Self-cleavage during transcription of the ELVd (+) hammerhead in the presence and absence of the antisense oligodeoxyribonucleotides (lanes 1 and 2, respectively), and of the mutant ELVd-(+)-GUC hammerhead (in which the wild-type AUA trinucleotide preceding the self-cleavage site has been substituted by GUC) in the presence of the antisense oligodeoxyribonucleotides (lane 3). Lane 4, RNA markers of 65 and 55 nt. Aliquots of the reaction mixtures were analyzed by PAGE in 15% gels containing 8 M urea that were stained with ethidium bromide. Positions of the complete (C) primary transcript (60 nt) and of the resulting self-cleavage 3′ fragment (3′F) (51 nt) are indicated with arrows. The self-cleavage 5′ fragment (9 nt) migrated out of the gel.