Figure 1.

Western blot analysis of (A) Cox-2, (B) TNF-α, (C) IFN-γ, and (D) IgG expression in arthritic joints of GTP-fed and non-GTP-fed DBA/1 mice. Arthritic joints were dissected free of soft tissue, and the cell-free extracts were prepared and analyzed as described in Materials and Methods. Results clearly show that the expression of Cox-2, TNF-α, IFN-γ, and mouse IgG was higher in the arthritic joints of non-GTP-fed DBA/1 mice and correlates with the clinical severity of the disease. Data from a representative experiment are shown. Similar results were obtained in a repeat Western blotting with samples obtained from a second independent experiment.