Fig. 4.

Photoreactivation of thymine dimer by Sso7d. (A and B) HPLC profile of ODN4 before (A) or after (B) photoirradiation in the presence of Sso7d. The reaction mixture (total volume, 10 μl) contained ODN4 (10 μM strand concentration) and Sso7d (20 μM) in 5 mM sodium cacodylate buffer (pH 7.0). Irradiation was performed with a monochrometor (295 nm) for 1 h at 0°C. The HPLC conditions were the same as described in Fig. 2 except for the acetonitrile gradient (3–10% over a linear gradient for 30 min). (C) Amount of ODN3 from photoirradiated ODN4 (10 μM) with Trp (W), Lys-Trp-Lys (KWK), or Sso7d (each concentrations are 5 μM).