Fig. 5.

Fluorescence of damaged tryptophan residue of Sso7d after photoirradiation. (A) Fluorescence emission spectra of photoirradiated Sso7d with (pink) or without (light blue) ODN2 (excitation 295 nm, 5-nm slit width) are shown. (B) Fluorescence emission spectra (excitation 319 nm, 10-nm slit width) of photoirradiated Sso7d with different concentrations of ODN2 are shown. (C) Amount of undamaged Trp in photoirradiated Sso7d with different concentrations of ODN2 (pink rectangles) and ODN3 (blue diamonds) is shown. These values were determined from the fluorescence intensity at 360 nm of each photoirradiated sample (excitation 295 nm). (D) Fluorescence emission spectra of photoirradiated Sso7d with or without ODN4 is shown. Photoreaction procedure for A-C and D are same as in Figs. 2 and 4, respectively, except the reaction volume of Sso7d-ODN4 was 20 μl. For quantification of Trp-24, the reaction mixtures were diluted to 50 μl with a NaCl solution (final concentration of NaCl was 3 M).