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. 2006 Oct 30;103(45):16722–16727. doi: 10.1073/pnas.0606482103

Fig. 3.

Fig. 3.

SDS/PAGE analysis of disulfide mutants. Samples (oxidized or reduced as indicated) were separated on a 10% NuPAGE Novex Bistris denaturing gel. (A) Mobility shift of disulfide-bonded mutants (lanes 4–5) compared with the parent protein (σ28-3M, which contains no cysteines, lane 1) or reduced mutants (lanes 2–3). (B) Analysis showing proportion of disulfide-bonded species after incubation under equilibrium conditions (see Materials and Methods). “R” indicates incubations that started with reduced samples, and “O” indicates incubations that started with oxidized samples.