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. 2006 Oct 31;103(45):16806–16811. doi: 10.1073/pnas.0603916103

Fig. 2.

Fig. 2.

Gene-expression analysis of regions of the PS and CD4-Foxa2+GFP-Bry+ EB subpopulations. (A) An embryonic day 7.25 mouse GFP-Bry embryo expressing GFP in the PS. Boxes indicate the posterior (Pos), middle (Mid), and anterior (Ant) PS regions. (B) RT-PCR gene-expression analysis of the different regions of the PS. (C) The presort (Pre), CD4loGFP+ (CD4 Lo), CD4medGFP+ (CD4 Med), and CD4hiGFP+ (CD4 Hi) populations were isolated by cell sorting from day 3.25 serum-stimulated EBs. (D) RT-PCR gene-expression analysis of the CD4 Lo, CD4 Med, and CD4 Hi populations.