Fig. 1.

HDAC inhibition attenuates denervation-dependent Mgn gene induction. (A and C) Relative Mgn RNA level in innervated and 14-day (A) or 3-day (C) denervated sternomastoid muscles bathed, in vivo, in buffer or HDAC inhibitors (HDACi) for 12 h before tissue harvest (n = 2, for each individual experiment). Error bars are standard deviation. (B) Western blot analysis of Mgn protein levels in innervated and 14-day denervated sternomastoid muscle bathed, in vivo, in buffer or NaB for 12 h before tissue harvest. (D) RNA levels in adult mouse diaphragm muscles placed in organ culture with TSA or vehicle for 48 h (n = 4). Error bars are standard error of the mean. All RNA levels were assayed by real-time PCR and normalized to γ-actin, which did not change after muscle denervation or HDAC inhibition. I, innervated; D, denervated.