Expression of sialyl 6-sulfo Lewis X and related carbohydrate determinants on a cultured human endothelial cell line, ECV304, transfected with 6-Sul-T and/or Fuc-T VII. ECV304 cells transfected with 6-Sul-T and/or Fuc-T VII were analyzed in flow cytometry by using anti-carbohydrate mAbs with well defined specificity. (Left) The carbohydrate structure recognized by the respective antibodies G152, G72, 2H5, and CSLEX-1.