Figure 3.

Reconstitution of L-selectin ligand activity on a cultured human endothelial cell line by transfection of 6-Sul-T and Fuc-T VII. (Top) Results of triplicate cell adhesion experiments using L-selectin transfectant clone of murine B lymphoma cells 300.19/L-selectin. (Middle and Bottom) Adhesion of 300.19/P-selectin and 300.19/E-selectin to these transfectant cells. HUT102-N cells, a dish-adherent subclone of cultured human lymphocytic leukemia HUT102 cells, served as positive control in these experiments. For experimental details, see Materials and Methods.