Figure 1.

Essential role of DAG in Ang II-induced cardiomyocyte hypertrophy. (A) Nuclear translocation of GFP-NFAT4 by Ang II stimulation. A portion of cells was treated with RHC80267 (30 μM) or xestospongin C (XestC, 20 μM) for 30 min before the addition of Ang II (100 nM), and a portion of cells was infected with DGKβ for 48 h before Ang II stimulation. (B, C) Quantification of nuclear predominant fluorescence of GFP-NFAT4 after Ang II stimulation. (D, E) Effects of DGKβ, RHC80267, and XestC on the increase in NFAT-dependent luciferase activity by Ang II stimulation for 6 h. The fold activation was calculated by the values of untreated cells set as 1. (F–H) Effects of DGKβ and GFP-IP₃-sponge on Ang II-induced actin reorganization (F), protein synthesis (G), and BNP expression (H). Scale bar=20 μm. ^*P<0.05, ^**P<0.01 versus control or LacZ-expressing cells.