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. 2006 Oct 26;25(22):5317–5328. doi: 10.1038/sj.emboj.7601404

Figure 1.

Figure 1

Sumoylation of MBD1 protein in vivo. (A) The anti-MBD1 antibodies detect an additional 130 kDa band in nuclear extracts prepared with SUMO isopeptidase inhibitor NEM. (B) The anti-MBD1 antibodies recognize a 130 kDa band in SUMO1 but not in SUMO2/3 IPs. (C, D) The 130 kDa band in MBD1 IPs is recognized by anti-SUMO1 but not by anti-SUMO2/3 antibodies. (E) SUMO1 siRNA efficiently reduces the ladder of SUMO1-modified proteins in HeLa nuclear extracts. An arrowhead indicates the major SUMO1-modified protein RanGAP1. (F) siRNA against SUMO2/3 leads to substantial loss of SUMO2/3-modified proteins in HeLa nuclear extracts. (G) SUMO1 but not SUMO2/3 siRNA reduces the sumoylation MBD1.