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. 2006 Nov 2;25(22):5250–5259. doi: 10.1038/sj.emboj.7601407

Figure 2.

Figure 2

Expression of dominant-negative Rab14 overcomes phagosome maturation block imposed by live mycobacteria. Macrophages were transfected with the indicated Rab14 constructs for 24 h, and infected with live Texas Red labeled BCG for 10 min. After 1 h, chase cells were fixed, stained for CD63 and examined by scanning confocal fluorescence microscopy. (A–U) Note colocalization between live BCG (red) and CD63 (blue) in cells transfected with Rab14S25N (M–P) and Rab14N125I (Q-T) as opposed to Rab14WT (E–H) and Rab14Q70L (I–L). (U) Quantification of colocalization between CD63 and live mycobacteria in cells transfected with Rab14 constructs. Bars, means±s.e.m. (3 independent experiments); n, number of phagosomes counted per condition. **P=0.007 (S25N), 0.0008 (N125I); †P=0.83 (Q70L), 0.77 (EGFP).