Figure 1. Fruiting in a and α Isolates Is Similar.

The filamentous isolate B3502a was cultured on V8 medium in the presence of synthetic α pheromone at 22 °C in the dark for two wk. The filamentous edge far from the original yeast growth patch was separated, fixed, and stained with DAPI.

(A) The hyphae produced are monokaryotic with unfused clamps.

(B) Four long chains of basidiospores were produced during fruiting.

(C) Basidia with one, two, or four nuclei at different stages of basidium development were observed.

In (A) and (C), the upper panel shows the DIC images, and the lower panel shows the corresponding fluorescent images.

Scale bar, 10 μm.