Translated amino acid sequences for Mac1 from strains B3501α and JEC21α (congenic to B3502a) are depicted. CNG02270 contains a copper-fist DNA binding domain at the N-terminus that is conserved between copper-regulating transcription factors, such as Mac1 of S. cerevisiae and Cuf1 of S. pombe. It also shares a copper-binding motif at the C-terminal region with S. pombe Cuf1 (328–342aa) that is important for activity.
^, sequences underlined with this mark are the predicted copper-Fist DNA binding domain.
#, cysteine and histidine residues conserved between Cuf1 of S. pombe and Mac1 of C. neoformans.
Cn, C. neoformans; Sc, S. cerevisiae; Sp, S. pombe.