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. 2006 Nov 17;2(11):e187. doi: 10.1371/journal.pgen.0020187

Figure 12. Mac1 Is a QTG Regulating Growth, Melanization, and Filamentation of C. neoformans .

Figure 12

Wild-type strain XL304α and its mac1 mutant were cultured on YPD medium with the indicated concentrations of BCS or copper sulfate at 22 °C (A) and 39 °C (B). The mac1 mutant showed hypersensitivity under copper-limiting and copper-rich conditions at both temperatures. Wild-type and the mac1 mutant were cultured on L-DOPA medium with the indicated concentrations of BCS or copper sulfate at 22 °C for induction of melanin production (C). The melanin production in mac1 mutant is also hypersensitive to copper in the medium, showing a bell-shaped response similar to growth (C). Wild-type and the mac1 mutant were cultured on V8 medium (pH 7.0) with the indicated concentrations of BCS or copper sulfate at 22 °C for induction of filamentation (D). Filamentation of the mac1 mutant is highly sensitive to copper ions in the medium (D). MAC1 allele exchange indicates different functions of the two alleles (E). Wild-type XL304α, wild-type JEC21α, JEC21α mac1 mutant, and transgenic JEC21α mac1 mutant with the MAC1 XL304α allele were cultured on L-DOPA medium at 22 °C with the indicated concentrations of BCS or copper sulfate (E).