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. 2006 Nov;26(21):7991–7998. doi: 10.1128/MCB.00904-06

FIG. 1.

FIG. 1.

Endogenous zona pellucida glycoproteins colocalized in MVA. Zona pellucida-free normal growing mouse oocytes were immunolabeled with fluorescently conjugated anti-ZP2 (Alexa Fluor 488) and anti-ZP3 (Alexa Fluor 568) monoclonal antibodies and with antibodies to syntaxin-6 detected with a secondary antibody (Alexa Fluor 633). Endogenous ZP2 (A) and ZP3 (B) colocalized (C) in the juxtanuclear endoplasmic reticulum (arrowhead), MVA (arrows), and plasma membrane. Syntaxin-6, a TGN/secretory vesicle marker, was also present in the oocytes (D) and colocalized in MVA (arrows) with ZP2 and ZP3 (E). (F) Electron micrograph of an MVA labeled with an anti-ZP3 monoclonal antibody detected by protein A-gold (arrowhead). The MVA comprised multiple vesicles (asterisk) embedded in an amorphous material. Scale bars: 10 μm (A to E) and 0.5 μm (F).