FIG. 3.

Analysis of PRY3 transcript structure in the presence of RNA Pol II inhibitor. (A) Total RNA was isolated from wild-type cells treated or not treated with 1,10-phenanthroline (100 μg/ml) for the times indicated, followed by S1 nuclease assay as described in the Fig. 2 legend. (B) Levels of PRY3 transcript from panel A as detected by the 5′ oligonucleotide probe (P1; solid line with triangles) or as detected by the 3′ probe (P2; dotted line with diamonds) were individually quantified and shown as a fraction of the PRY3 transcript from cells at 0 min. Data are representative of four independent experiments. (C) Wild-type cells were treated with 1,10-phenanthroline (100 μg/ml) for 5 min prior to the addition of α-factor at 0 min. Total RNA was isolated from cells harvested and frozen at the times indicated, followed by S1 nuclease assay as described in the Fig. 2 legend. (D) Levels of full-length transcript (solid line with triangles) and +452 transcript (dashed line with squares) from panel C were quantified and shown as a percentage of the full-length transcript from cells at 0 min. Data are representative of three independent experiments.