FIG. 2.

Validation of the DNA array screen. (A) SYBR green real-time RT-PCR analysis of upregulated genes. Total RNA isolated from wild-type (WT) and HDAC1-deficient ES cells was reverse transcribed, and PCR was performed with primers specific for the cDNA of indicated genes. Gene expression levels were normalized to tubulin α1 levels and shown as expression levels in HDAC1-null cells relative to expression levels in wild-type cells (arbitrarily set to 1). (B) SYBR green real-time RT-PCR analysis of downregulated genes. The analysis was performed as described for panel A.