FIG. 2.

DIAP2 is required to resist gram-negative bacterial infection. The survival rates of adult males in response to different types of septic injuries are presented. Animals were pricked with a needle previously dipped into Erwinia carotovora subsp. carotovora 15 (Ecc15) (A), Enterococcus faecalis (E.faec.) (B), or Candida albicans (C.alb.) (C). The following genotypes were examined for susceptibility to microbes: wild-type (Oregon^R), Tak1¹, Relish^E20, spatzle^rm7, EP(G2326), diap2^7c/def, diap2^7c, diap2^7a, and diap2^7c/diap2^7a. Note that diap2 mutant flies behaved as Tak1 and Relish mutant flies, which are known to be highly susceptible to Erwinia carotovora subsp. carotovora 15 infection (A), but not to Enterococcus faecalis (B) or Candida albicans (C) infection. Both diap2 alleles, diap2^7c and diap2^7a, hemizygous (diap2^7c/def) or transheterozygous flies (diap2^7c/diap2^7a) showed similar susceptibility to E. carotovora subsp. carotovora 15 infection, while animals of the parental EP(G2326) line were fully resistant (A).