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. 2006 Aug 7;26(21):7821–7831. doi: 10.1128/MCB.00548-06

FIG. 3.

FIG. 3.

Ubiquitous expression of DIAP2 fully rescues the immune deficiency phenotype associated with diap27c. (A) Expression level of diap2 transgene in otherwise diap2 mutant flies. The presence of DIAP2 protein was examined by immunoblot analysis using anti-DIAP2 and antitubulin antibodies. Protein extracts from the following genotypes were used to monitor DIAP2 protein expression: CantonS (wild type [WT]) (lane 1), diap27c (lane 2), diap27c; Act5C-GAL4/+ (lane 3), diap27c; UAS-diap2/+ (lane 4), diap27c; UAS-diap2/Act5C-GAL4 (lane 5), and diap27c; UAS-diap2/Da-GAL4 (lane 6). Quantification of signals was performed using the LICOR system. (B) diap2 transgene expression rescued diap27c mutant flies from the lethal effects of Erwinia carotovora subsp. carotovora 15 (Ecc15)-mediated septic injury. The following genotypes were examined for susceptibility to microbes: wild type (OregonR), Tak11, diap27c, diap27c; Act5C-GAL4/+, diap27c; UAS-diap2/+, diap27c; Act5c-GAL4/UAS-diap2, and diap27c; Da-GAL4/UAS-diap2.