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Induction of early TCR signaling events in lck+ and lckW97A thymocytes. Single-cell suspensions of thymocytes were stimulated (stim.) with 3 μg/ml anti-CD3 for 3 min. (A) Lysates were analyzed by immunoblotting with antiphosphotyrosine antibody. (B and C) Immunoprecipitates were blotted with antiphosphotyrosine antibody, anti-ZAP-70, or anti-CD3, as indicated. Results are representative of at least three experiments.
