FIG. 6.
Dox-dependent recruitment of Notch1IC and MAM to the hes1 and c-myc promoters. (A) ChIP assay for CSL, Notch1IC, MAM, RNA polymerase II (Pol II), CDK9, and acetylated histones H3 and H4. Briefly, the Dox-dependent 3404 leukemic cell line was grown in RPMI medium with 10% fetal bovine serum at 37°C and treated with doxycycline (2 μg/ml) for 48 h (+Dox) or left untreated (−Dox). Immunoprecipitations (IPs) were carried out using the respective monoclonal or polyclonal antibodies or without antibody (No-ab) as a control. Immunoprecipitated and input DNAs were amplified by PCR using primers specific for the mouse hes1 promoter (A) or the mouse c-myc promoter (B and C). PCR-amplified products were analyzed on 2% agarose gels and stained with ethidium bromide.