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. 2006 Aug 28;26(21):8191–8201. doi: 10.1128/MCB.00242-06

FIG. 1.

FIG. 1.

AIB1 is a target of Mir-17-5p. (A) Sequence alignment and diagram of Mir-17-5p and its complementary sites in the AIB1 cDNA. The hatched area indicates the coding region; the black square indicates the region cloned at the end of the luciferase reporter gene. (B) Activity of luciferase reporters containing the Mir-17-5p complementary site from human AIB1 (LucMir-17-5p), a mutant Mir-17-5p complementary site from human AIB1 (mutLucMir-17-5p), or the perfect antisense sequence of Mir-17-5p (LucMir-17-5pAS). The reporters were cotransfected with the indicated amounts of 2′-O-methyl Mir-17-5p or Mir-95. Firefly luciferase activity was normalized to that of β-galactosidase. The data shown are the mean of three independent experiments, and error bars indicate the standard deviations. (C) Activity of luciferase reporters containing the perfect antisense sequence of Mir-95 (LucMir-95AS). The reporters were cotransfected with the indicated amounts of 2′-O-methyl Mir-17-5p or Mir-95. Firefly luciferase activity was normalized to that of β-galactosidase. The data shown are means of three independent experiments, and error bars indicate standard deviations.