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. 2006 Aug 28;26(21):8191–8201. doi: 10.1128/MCB.00242-06

FIG. 4.

FIG. 4.

Expression of Mir-17-5p is reduced or silent in some cancer cell lines. (A) Northern blot sequentially assayed with radiolabeled probes specific for Mir-17-5p, with U6 snRNA used as a control. Lanes 1 to 17 show RNA from the cell lines ZR-75-1, MCF-7, BT-20, MDA-MB-361, BT-474, T-47D, MDA-MB-468, MDA-MB-453, BG-1, Hs 748.T, BT-549, SW527, EB-3, HeLa, OV90, HEL60, and K562. Lanes 1 to 12 show breast cancer cell lines, and lanes 13 to 17 show other types of cancer cells. (B) Western blot of AIB1 protein in different cell lines. Cell lysates (50 μg) of the different cell lines were separated by SDS-PAGE, transferred onto nitrocellulose membrane, and probed with anti-AIB1 antibody. The same blot was stripped and reprobed with anti-β-actin antibody and was used as a loading control (bottom panel).