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Reduction of endogenous AIB1 by Mir-17-5p inhibits the anchorage-independent growth of MCF-7 Cells. (A) Mir-17-5p and Mir-95 overexpressing stably transfected MCF-7 cells were grown in improved minimal essential medium and 1% charcoal-stripped calf serum in 0.35% soft agar dishes in the absence or presence of 100 ng of IGF-1/ml. (B) Mean numbers of colonies with a diameter >80 μm. The data shown are means of triplicate experiments, and error bars indicate the standard deviations.
