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. 2006 Aug 28;26(21):7977–7990. doi: 10.1128/MCB.00819-06

FIG. 4.

FIG. 4.

Disruption of NER does not cause activation of cell cycle checkpoint signaling. (A) Cells were transfected with control, DDB1, or DDB2 siRNA oligonucleotides. Three days after transfection cell lysates were harvested, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotted with the indicated antibodies. (B) Checkpoint activation was monitored on days 2, 3, and 4 after transfection with control, DDB1, and XPC siRNA oligonucleotides by immunoblotting with the indicated antibodies. (C) Cells were transfected with control, DDB1, or XPA siRNA oligonucleotides. Three days after transfection, checkpoint activation was examined by immunoblotting with the indicated antibodies. (D) The efficiency of depletion by the XPA and XPC siRNA oligonucleotides was monitored by immunoblot analysis. The asterisks denote nonspecific, cross-reacting proteins.