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. 2006 Aug 21;26(21):7871–7879. doi: 10.1128/MCB.00573-06

FIG. 1.

FIG. 1.

Yng1p interacts with the histone H3 tail in vivo. (A) Chromatin pull-down assays were performed on strains YDM126, YDM127, and YDM138, and the resulting samples were subjected to Western blotting with peroxidase antiperoxidase (Htb1TAP) or anti-HA antibodies (Sas3HA). (B and C) Tenfold serial dilutions of yeast strains YLH101 (B) or FY2162 transformed with pHHT2 or phht2Δ3-29 (C) containing the indicated plasmids were plated on synthetic complete medium lacking uracil containing either dextrose or galactose as a carbon source and incubated at 30°C for 3 days. (D) Normalized amounts of whole-cell extracts from the indicated strains (FY2162 transformed with pHHT2 or phht2Δ3-29) transformed with pGALYNG1HA and grown on galactose were subjected to Western blotting with immunodetection for HA. +, present; −, absent.