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. 2006 Aug 21;26(21):7871–7879. doi: 10.1128/MCB.00573-06

FIG. 4.

FIG. 4.

The Yng1p PHD finger is required for histone binding. (A) Chromatin pull-down assays were performed on strains YDM126, YDM127, YDM137, YDM153, and YLH363, and the resulting samples were subjected to Western blotting with peroxidase antiperoxidase (Htb1TAP) or anti-HA antibodies (Sas3HA). (B) Tenfold serial dilutions of yeast strain YLH101 containing the indicated plasmids were plated on synthetic complete medium lacking leucine containing either dextrose or galactose as a carbon source and incubated at 30°C for 3 days. (C) Normalized amounts of whole-cell extracts from wild-type yeast strains (YLH101) carrying a pGALYNG1HA (lane 1) or pGALYNG1ΔPHDHA (lane 2) plasmid and grown on galactose were subjected to Western blotting for HA. +, present; −, absent.