FIG. 4.

Expression of mitochondrial marker proteins and DNA are similar in wild-type and Tyk2-null pro-B cells. (A) Cells (5 × 10⁶) from wild-type and Tyk2-null pro-B cells were lysed directly in sodium dodecyl sulfate extraction buffer and analyzed for the levels of porin and cytochrome c (mitochondrial markers) and α-tubulin (a cytosolic marker) using Western blot analysis. (B) DNA was isolated from wild-type and Tyk2-null pro-B cells. Quantitative PCR was performed to measure the levels of ND1, COX1, COX2, or ATPase6. Values were normalized to the actin gene, and wild-type mitochondrial transcripts were given a value of 1. m refers to the fact that the genes are present in mitochondrial DNA.