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Impaired T-cell proliferation and IL-2 secretion in the absence of Lck. Proliferation of CFSE-labeled, purified CD4+ T cells stimulated with anti-CD3 plus anti-CD28 (A) or PDBu plus ionomycin (B) for 72 h. (C) IL-2 was measured from supernatant after 24 h culture, in response to anti-CD3 plus 1 μg/ml anti-CD28, and (D) expression of CD25 was determined. Graphs show means ± standard deviations for triplicate points.
