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RNAi against endogenous AUF1 stabilizes GFP-IL-6 mRNA. (A) Western blot analyzed with polyclonal anti-AUF1 and anti-γ-tubulin antibodies after expression of three different interfering RNAs in mouse NIH 3T3 cells. Oligo, oligonucleotide. (B) Half-lives of GFP-IL-6 mRNA measured by the Tet-Off system before and after infection with a retroviral RNAi vector carrying the oligonucleotide 301 sequence, which targets AUF1 exon 5.
