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Submaximal insulin stimulation of Akt Thr308 phosphorylation. EDL and soleus muscles were dissected and incubated with submaximal insulin (450 μU/ml). (A and B) Insulin-stimulated Akt Thr308 phosphorylation in EDL and soleus muscle was greater in MLKB1KO mice than in controls. Data are means ± SEM; n = 5 to 6/group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus basal level of same genotype. †, P < 0.05 versus results with corresponding +/+ mice.
