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Results from TRE trap assays. (A) In this experiment 4 × 106 D. discoideum cells carrying the empty trap (−), a D. discoideum Glusup gene, or a D. discoideum ValUAC gene were subjected to selection in 5-FOA and uracil. (B) Comparison of targeting frequencies at a human MetCAU gene and a D. discoideum ValUAC gene. D. discoideum cells (5 × 106 of each strain) were used for 5-FOA selection. Mean clone numbers from 10 petri dishes are shown ± SD.
