FIG. 9.

5′ junctions of new TRE5-A integrations. (A) Targeting of the D. discoideum r5S gene. The target sequence is written in the first line in lowercase letters. The 5′ end of the r5S gene (reverse orientation) is shown in uppercase letters. The vertical arrow points at the integration site of a TRE5-A element; the numbers indicate the distance of the TRE5-A to the first nucleotide of the targeted r5S gene. The numbers in parentheses indicate the first nucleotides of the inserted TRE5-A, which are written in bold uppercase letters. Target site deletions are indicated as “Δ.” (B) Integrations of TRE5-A upstream of an r5S tandem. The first line indicates the target sequence, which consists of two r5S genes (uppercase) separated by an EcoRI restriction site and the transcription terminator sequence of the first r5S gene (lowercase). The first nucleotides of the inserted TRE5-As (bold uppercase) are written in parentheses; extra nucleotides are boxed.