FIG. 5.
Mutant MYOC does not activate the unfolded protein response. (A) Western analysis of lysates from a tissue ring enriched with iridocorneal drainage structures did not reveal elevated levels of the chaperone GRP94 levels in MyocY423H/+ or MyocY423H/Y423H mice compared to control (Myoc+/+) mice. (B) Amplification of cDNA with XBP-1-specific primers did not reveal the presence of processed XBP-1 transcripts. IRE1 is a proximal transducer of the UPR. IRE1 processes XBP-1 RNA to generate an XBP-1 transcript whose protein activates a number of UPR target genes. Together, these data suggest that the unfolded protein response is not activated by the presence of mutant MYOC protein in vivo.